CHARACTERIZATION AND PURIFICATION OF UREASE ENZYME FROM NEW PROTEUS MIRABILIS STRAIN

Abstract

Proteus mirabilis bacterium is a common etiologic agent of urinary tract infections and produces large amounts of urease enzyme (EC 3.5.1.5). The urease enzyme producing new Proteus mirabilis isolate was isolated from positive sample collected from Khartoum hospital. In this study’s the enzyme was extracted from the bacterial isolate by distilled water and purified by gel-permeation. Urease enzyme activity was measured in SDS page based on cresol red and assayed spectrophotometically. The recovery percentage of pure urease enzyme was in range 55-98%. The Bacterium protein extraction retained urease enzymatic activity and showed the typical subunits of Mr 66000, 45000, 29000, and 15000 when subjected to sodium dodecyl sulfatepolyacrylamide gel electrophoresis.