EVALUATION OF ROSMARINUS SPECIES EXTRACTED BY DIFFERENT SOLVENTS AGAINST MOSQUITO LARVAE, BIOMOPHALARIA SPECIES AND DIFFERENT PATHOGENIC BACTERIA

Abstract

The botanical extracts were used as a scours of insecticidal, molluscicidal and antimicrobial agents. Many authors try to improve the potency of these extracts by using different solvents. The pet-ether and ethanolic extracts of Rosmarinus species were tested as insecticidal and molluscicidal agents against Culex pipiens and Biomophalaria alexandrina. The LC50 of pet-ether and ethanolic extracts against mosquitoes were 511.84 8 721.81 ppm respectively. While, LC50 of these extracts against snails were 236.81 8 276.16 ppm respectively. From those results the snails appear more susceptible to both extracts than mosquitoes. Both mosquitoes and snails more susceptible to pet-ether extract than ethanolic extract. petroleum ether and ethanol extracts of Rosamarinus sp in different concentrations (5, 10, 15 and 20%) were evaluated for their possible antibacterial activity against six pathogenic bacteria, Staphylococcus aureus and Entrococcus faecalis (gram positive bacteria), Escherichia coli, Proteus sp, Acetobacter sp and Pseudomonas aeruginosa (gram-negative bacteria). The petroleum ether and ethanol extracts of Rosamarinus sp. in different concentrations exhibited antibacterial activity against all tested organisms (except Acetobacter sp was resistant at all concentrations of petroleum ether extract). Findings were compared to those produced by Gentamycin (10 μg) and Tetracyclin (30 μg), reference antibiotic.