SPECTROGRAPHIC ANALYSIS AND IN VITRO STUDY OF ANTIBACTERIAL, ANTICANCER ACTIVITY OF AQUEOUS ETHANOLIC FRUIT EXTRACT OF CARISSA CARANDAS L.

Abstract

Fruits are the richest source of nutrients and minerals. Carissa carandas belongs to the Apocynaceae family and widely spread throughout India. Spectrographic methods are most reliable, for the analysis of phytochemicals. Carissa carandas fruits are plentiful sources of phytochemicals and possessing beneficial biological activities. The present work was first to focus on analysing of such phytochemicals in aqueous ethanolic fruit extract (AEE) using UV-Visible and Fourier transform infrared spectroscopy (FTIR). The fingerprint of the UV-spectra showed maximum absorption peak at 270 nm and FTIR helps in the recognition of functional groups and assignments. Furthermore, in-vitro studies showed that AEE possesses lowest MIC value 0.3125 mg/ml and induces cytotoxicity at 800μg/mL on HeLa cancer cells. The study concludes that, regular intake of fruits in daily diet suggested with reduced risks of infectious diseases and cancer.