DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR DETERMINATION OF RIFABUTINE IN API AND CAPSULE DOSAGE FORM

Abstract

A simple, rapid, selective and quantitative HPTLC method has been developed and validated for determination of Rifabutine in bulk and capsule dosage form. The Rifabutine samples were applied on TLC aluminium plate pre coated with Silica gel60 GF254 and developed using acetone as a mobile phase. The bands were scanned at λ=282 nm using Camag TLC scanner 3 and detection and quantification were carried out densitometrically using an UV detector. The Rf value was found to be 0.58. The linearity of the method was found to be within the concentration range of 100-700 ng/spot and its percentage recovery was found to 97.89 %. The limit of detection and the limit of quantification were found to be 36.83 ng/spot and 111.6 ng/spot respectively. The Correlation of determination (r2) was 0.9995. The regression equation was found to be y = 11.906x+856.83. The method was also validated for precision, specificity and recovery. This developed method was used to analyze marketed formulation.