Development of Validated Spectrophotometric Method for Simultaneous Estimation of Acetylsalicylic Acid and Caffeine in Pure and Tablet Dosage Form

Abstract

A sensitive, rapid, and specific spectrophotometric assay method has been developed for the simultaneous determination of acetylsalicylic acid and caffeine in commercial tablets. Acetyl salicylic acid and caffeine showed absorbance maxima at 297 nm and 272 nm respectively in 0.1N NaOH solution. These drugs were estimated in formulations by simultaneous equation method (method A) and absorbance ratio method (method B). In method A, acetylsalicylic acid and caffeine at their respective λmax of 297.0 nm and 272.0 nm showed linearity in the concentration range of 0-40 μg/mL and 0-25 μg/mL. Method B involved measurement of absorbance at isoabsorptive point of these two drugs i.e. at 289 nm with linearity in the concentration range of 0-40 μg/mL and 0-25 μg/mL for acetylsalicylic acid and caffeine respectively. Validation study revealed that the methods are specific, accurate, precise, and reproducible. The developed methods are simple, rapid, accurate, precise, reproducible, and economic and can be used for routine quantitative analysis of acetyl salicylic acid and caffeine in pure and tablet dosage form.