IN VITRO CALLUS INDUCTION AND HIGH FREQUENCY SHOOT REGENERATION FROM LEAF EXPLANTS OF SOLANUM PUBESCENS WILLD

Abstract

An efficient protocol for the shoot regeneration of Solanum pubescens Willd. using leaf derived callus induction has been described. The optimum callusing was observed when leaf explants were cultured on MS medium augmented with 3.0 mg/l 6- benzylaminopurine (BAP) 1.0 mg/l 1-Napthaleneaceticacid (NAA) and 0.5 mg/l Indoleacetic acid (IAA). The couple were subcultured on MS medium supplemented with various concentrations of (BAP 3.0 mg/l), kinetin (KIN 1.2 mg/l) alone or in combination with Gibberellic acid (GA3 0.5 mg/l) for shoot regeneration. The highest shoot regeneration in terms of percent response was found to be 99%. The regenerated shoots were transferred in to half strength MS medium fortified with indole-3- butyric acid (IBA) for root induction. Rooted plantlets were successfully acclimatized. The protocol described here could be used for the mass multiplication and conservation of this plant.