PRODUCTION OF LACCASE BY GANODERMA Sp. IN SUBMERGED FERMENTATION

Abstract

The present study focuses on enhancing the process parameters to achieve the maximum production of extracellular laccase by Submerged Fermentation (SmF). Culture conditions and medium composition play a major role in enzyme expression. Laccases are generally produced during the secondary metabolism of white-rot fungi growing on natural substrate or in submerged culture. Physiological demands vary among white-rot fungi and considerable research has been done on the influence of agitation, pH, temperature, carbon, nitrogen sources, microelements and their levels. Submerged fermentation involves the nurturing of microorganisms in high oxygen concentrated liquid nutrient medium. The production parameters production medium, pH, temperature, solvents, carbon and nitrogen sources were studied. Yeast extract peptone dextrose-Copper sulphate (YPD-Cu) medium good supporter for production of laccase. pH and temperature for highest laccase activity was found to be 6.0 and 4ºC respectively. Starch and peptone were the most suitable carbon and nitrogen sources for maximum laccase production. Ethanol was the most suitable solvent for laccase production.