GENOME ANNOTATION OF UNCHARACTERISED PROTEINS IN NEISSERIA MENINGITIDIS K1207

Abstract

The origin of high-throughput biology has catalyzed a phenomenal improvement in our ability to identify new proteins. Structural and functional characterization of Hypothetical proteins reveal vital roles in bacteria, especially in pathogens related to human diseases. Neisseria meningitidis strain K1207 is a diplococcus Gramnegative bacteria that possesses enzymes that play important role in many cellular mechanisms. However, from its genome many proteins are Hypothetical or un-annotated. These functionally unknown proteins may specify important functions concerning biological role of this bacterium. By using bioinformatics tools the biological understanding of the organisms easily done through functional annotation analysis. There were different bioinformatics tools used for annotation- NCBI, MOTIF SCAN, INTERPRO, BLAST, TMMOD, CRNPRED, SOSUI, PROTPARAM, PSLPRED, PHYRE2. The sequence of 130 Hypothetical proteins were submitted to this workflow resulting in successful identification of proteins based on several parameters. Our study helps to found out two proteins at last, these proteins possess confidence score above 90%. These proteins play important roles in aerobic and anaerobic growth of gram negative bacteria and also in various signaling pathways. Data obtain by bioinformatics tools might facilitate swift identification of prospective therapeutic targets and thereby enabling the search for new inhibitors or vaccines. Our research unlock the chance to analyze the various applications of these functionally predicted proteins in area of biotechnology.