HPTLC ANALYSIS AND ANTIMICROBIAL ACTIVITY OF ETHYL ACETATE EXTRACT OF SEA URCHIN (ARBACIA LIXULA) SHELL

Abstract

The study aimed to find out the phytochemical expression in shell of sea urchin Arbacia lixula by using the solvent ethyl acetate. The ethyl acetate extract was obtained and the presences of phytochemical or micro molecules were found by the HPTLC. Further, the extract was analyzed for its antimicrobial activity against the human pathogens both bacteria and fungi. The growth inhibition of bacteria and fungi was determined using the solvent extract and where the ability was compared with commercial available drugs. The minimum inhibitory concentration of the solvent extract was found against the bacteria and fungi used. As a result, the HPTLC showed 5 spot with different Rf values which shows the shells of sea urchin also rich with phytochemicals or micro molecules.