PHYTOCHEMICAL SCREENING AND IMMUNOMODULATOR ACTIVITY OF GREWIA ASIATICA LINN. LEAVES

Abstract

The leaves of Grewia asiatica were extracted by Petroleum ether and then Ethanol till all the constituents were separated out. Phytochemical screening of Ethanolic extract of Grewia asiatica reveals the presence of Flavonoids, Alkaloids, Glycosides, Saponins, Tannins, Carbohydrates, and Phenolic compounds. To find out the Immunomodulator properties of the extract, different parameters of Immunomodulator were performed on Swiss albino rats, like Carbon Clearance study and Phagocytic index, Delayed type of hypersensitivity reaction, Albumin : Globulin ratio, Sheep red blood cell agglutination test, Estimation of Immunoglobulin, and Total leukocytes count. The results obtained with various experiments suggested that the Ethanolic extract of Grewia asiatica leaves exhibited immunomodulatory properties with satisfactory immunostimulation. Preliminary Phytochemical screening of the extract found to contain Flavonoids. It is reported that flavonoids and their derivatives also contribute to macrophage activation. Hence, the presence of flavonoids may contribute to immune stimulating activity.