UV-Spectrophotometric Methods For The Determination Of Zolmitriptan in Bulk and Pharmaceutical Dosage Forms

Abstract

UV, first derivative, and AUC-spectrophotometric methods for the determination of zolmitriptan in bulk and pharmaceutical formulations have been developed. For the UV-spectrophotometry, standard solutions were measured at 283.0 nm. The linearity ranges were found to be 0.5–100 μg/mL in 0.1M HCl and the regression equation was A=2.02×10−2C+4.6×10−4 (r2=0.9999). For first derivative spectrophotometry, the response (dA/dλ) of standard solutions was measured at 298.0 nm. Calibration curve was constructed by plotting dA/dλ values against concentrations, 1–100 μg/mL of zolmitriptan. Regression equation of linear calibration graph was calculated as D1= -1.14×10−3C-2.00×10−5 (r2=0.9999). The AUC-spectrophotometric method was based on calculation of area under curve (AUC) for analysis of zolmitriptan in the wavelength range of 278.0–288.0 nm. Calibration curve was constructed by plotting AUC values against concentrations, 0.5–100.0 μg/mL of zolmitriptan. Regression equation of linear calibration graph was calculated as AUC=1.963×10−1C+1.34×10−3 (r2=0.9999). The methods were validated by following the analytical performance parameters suggested by the International Conference on Harmonization. All validation parameters were within the acceptable range. The developed methods were successfully applied to estimate the amount of zolmitriptan in pharmaceutical formulations.