A TEMPORARY STABILITY AND VERTICAL LACTATING MOTHER-INFANT TRANSFER OF BREAST MILK MICROBIOTA THROUGH BREASTFEEDING

Abstract

Human milk consists of a diverse microbial community i.e. human-friendly probiotics and commensalbacteria. The current study explored whether viable breast milk microbes are shared between the maternal and infant gut ecosystem via breastfeeding. Healthy lactating mother milk and corresponding neonatal faeces collected from five mothers-neonate pairs at every week's time interval were studied by culture-dependent methods (16S rRNA gene sequencing). Bythe culture-dependent method, a viable isolated strain of Lactobacillus oris, Lysinibacillus sp., Enterococcus mundtii, Bacillus clausii, Enterococcus faecalis, Lactobacillus brevis, Lysinibacillus fusiformis, and Staphylococcus sp. was revealed to be shared among both ecosystems within mother-neonate pair. This study shows that viablemicroflora may be vertically transmitted from mother to infantthrough breastfeeding. Hence, our data support the proposed hypothesis of a newpathway of motherinfant communication, in thatmother gut microflora reaches breast milk by an entero-mammary pathway to develope neonatal gut microflora and maturation of the immune system.