ANTIBIOFILM AND ANTIQUORUM SENSING POTENTIAL OF CUMINUM CYMINUM AGAINST AEROMONAS VERONII

Abstract

Bacteria have the capacity to communicate with their population densities through a mechanism called Quorum sensing. They can interfere with bacterial virulence and can produce signaling molecules called Auto inducers (AI). The high amount of signaling molecules induced pathogenicity and can cause biofilm formation. Aeromonas veronii can produce natural AHL (N-Acyl homoserine lactones) and can lead to quorum sensing functions. Cumin, a traditionally used medicine containing compounds like tannins, terpenoids, phenols, saponins, steroids, flavonoids, carbohydrates, proteins and amino acid acted as anti-quorum sensing inhibitor. Biofilm formation were assayed by methods like tube assay, Congo red agar and microtiter plate method and they have shown strong biofilm formation and also Aeromonas veronii exhibit exopolysaccharide production. Test organism when treated with Cumin extract resulted 3.12mg/mL as the lowest MIC value and 6.25mg/mL as the MBC value. The highest anti-quorum sensing value of cumin ranged at 25mg/mL and 50mg/mL concentrations. Higher concentration of seed extract exhibited low biofilm formation. The results of DPPH assay showed cumin has 99% of antioxidant activity. The present study revealed that the methanolic extract of cumin inhibited quorum sensing activity by interfering AHL production and thus inhibited biofilm formation of organism.