FORMULATION AND CHARACTERIZATION OF SOLID LIPID NANOPARTICLES OF RIFAMPICIN

Abstract

The purpose of this work was to develop prolonged release solid lipid nanoparticles (SLNs) of Rifampicin (RIF) for oral drug delivery and to improve the bioavailability of RIF. SLNs were designed by using cetyl palmitate (CP) and as lipid core materials and polaxamer 188 as stabilizer. SLNs were prepared by o/w microemulsion technique and characterized by particle size analysis, Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC), drug entrapment efficiency, scanning electron microscopy (SEM), Zeta potential, in vitro evaluation studies, storage stability, membrane permeation study. At highest speed the resultant SLNs were smaller in size and their size increased with increase in lipid concentration. Nanoparticles prepared using 5.0% lipid, 1.5% Poloxamer 188 and 9,500 rpm shows smaller particle size, better drug entrapment, excellent surface characteristic and controlled drug release. Release from RIF-SLNs was studied using a dialysis bag method. The results of in-vitro release study shows that the dialysis membrane after 12 and after 24 h the release pattern of drug slightly increases. The surface characters were found to be smooth with all the lipid carriers. Short term stability studies indicated no significant change, when stored between 2-8˚C for one month.