PHYTOCHEMICAL SCREENING, GC-MS ANALYSIS, ANTIOXIDANT ACTIVITY AND IN VITRO ANTICANCER ACTIVITY OF LEAF EXTRACT OF SEMECARPUS ANACARDIUM. LINN (ANACARDIACEAE)

Abstract

The aim of the present study was to screen the phytochemicals, characterize the active bio components from the acetone leaf extract of Semecarpus anacardium Linn through GC-MS analysis and to further study their inhibition percentage against the reactive oxygen species using Nitric oxide radical scavenging assay. Cytotoxic activity was also carried on the leaf extract against Vero cells and HepG2 cells using MTT assay. The phytochemical screening showed the presence of various phytochemicals. The GC-MS analysis revealed the presence of ten bioactive components. The antioxidant activity of S. anacardium leaf extract showed an inhibition percentage of 42.28 ± 0.069 at 5 μg/ml and 53.03 ± 0.069 at 25 μg/ml respectively while that of the standard ABA showed 47.04 ± 0.069 at 5 μg/ml and 56.79 ± 0.069 at 25 μg/ml, both showing a significance value less than one. The in-vitro anticancer activity of S. anacardium on normal cell lines and liver cancer cell lines (HepG2) showed a decline in cell viability percentage with the increase in sample concentration.