NEUROPROTECTIVE EFFECT OF HESPERIDIN ON 6-OHDA INDUCED PARKINSONISM IN SHSY5Y CELLS

Abstract

Parkinson’s disease (PD) occurs due to oxidative stress and it is the second most common neurodegenerative disorder after Alzheimer’s disease. 6-OHDA (6-hydroxydopamine) is a widely used neurotoxin for investigating the pathology of PD both in vitro and in vivo conditions. Studies have shown that the neurotoxicity of 6-OHDA may be related to its ability to generate reactive oxygen species (ROS) leading to neuronal damage. The current study was carried out to investigate the neuroprotective effects of hesperidin, a citrus fruit flavanol, against 6-OHDA induced neurotoxin in human neuroblastoma SHSY5Y cells. We assessed cell viability by MTT assay, ROS production by DCFH-DA and Nuclear change by DAPI. Cell death was determined in normal, 6-OHDA and hesperidin treated cells. The cell death in 6-OHDA induced SHSY5Y cells was accompanied by the loss of neurons, increased ROS generation, the depletion of GSH, enhanced activities of enzymatic antioxidants which were attenuated in the presence of hesperidin. Our data suggests that hesperidin exerts its neuroprotective effect against 6-OHDA due to its antioxidant properties in a neuroblastoma SHSY5Y cell lines.