α -AMYLASE, α -GLUCOSIDASE AND ALDOSE REDUCTASE INHIBITORY POTENTIAL OF BETANIN FOR THE MANAGEMENT OF DIABETES AND ITS COMPLICATIONS

Abstract

Diabetes is an important human ailment that affects many countries. It's proving to be a major health problem in India, especially in urban areas. Herbal formulations are used to reduce the ill effects of diabetes and its complications. The present study demonstrates the potential of Betanin; a natural compound obtained from Beta Vulgaris, against the enzymes aldose reductase, α-glucosidase, and α-amylase, involved in diabetes and its complications by in vitro evaluation. Aldose reductase assay was performed by using NADPH as starting material and DL-Glyceraldehyde as a substrate. Ranirestat was used as standard drug. DNS method was used for alpha amylase inhibitory activity and for alpha glucosidase inhibitory activity, p-nitrophenyl glucopyranoside (pNPG) was used as substrate. Acarbose was used as a standard drug for alpha amylase and alpha glucosidase enzymes. Betanin shows potent inhibitory effect against aldose reductase (IC50:4.245±0.068μg/ml), α-glucosidase (IC50:2.85±0.0219μg/ml) and α-amylase (IC50:1.75±0033μg/ml), respectively. From the results it was concluded that Betanin potentially inhibit all the three enzymes more than their standard drugs and it will be helpful in the management of diabetes and its complications.