CYTOTOXICITY OF NANOPARTICLE ON THE SEEDLING OF THE PLANTS BELONGING TO THE FABACEAE FAMILY

Abstract

The research was carried out to show the influence of Silver, Iron and Sulphur Nanoparticles on seed germination under in-vitro condition on different morphological and biochemical parameters. In the present study, Silver (AgNPs), Iron nanoparticles (Fe NPs) and Sulphur nanoparticles (SNPs) were synthesized by chemical reduction process. UV-vis spectrophotometer analysis revealed that synthesized AgNPs, FeNps and SNPs are in nano size range and the influence of synthesized NPs were studied on germination and seedling growth of Vigna radiata (Green Gram). Seeds of samples were surface sterilized using ethanol 90%, soaked in a sterile distilled water for 60min, then soaked in silver, Iron and Sulphur nanoparticles at 100 mg/L (100 ppm) concentrations for 15 minutes. The work was initiated from treated and nontreated seed embryos of sample. The effect of nanoparticles on the morphological and biochemical level was detected. Protein, amino acid, sugar, Tannin, alkaloid and flavonoid content were used as biochemical parameters to estimate the effects of AgNPs, FeNP and SNPs on the metabolism of seedlings. The Results indicated that nanoparticles positively influenced the growth of the plants for most of the parameters studied, such as seed germination, root growth, biochemical metabolism of plants i.e. primary and secondary metabolites.