COMPARATIVE PHYTOCHEMICAL INVESTIGATION AND DETERMINATION OF FLAVONOIDS, ALKALOID AND ANTIOXIDANT ACTIVITY OF LEAVES, STEM AND FLOWER EXTRACT OF PYROSTEGIA VENUSTA

Abstract

Medicinal plants include bioactive substances used to treat different human diseases, and they often play a significant part in various treatment. Secondary materials include alkaloids, flavonoids, phenol, saponin, tannins, and steroids. Medicinal plants have properties such as anticancer, antimicrobial, antidiabetic, antidiuretic and anti-inflammatory, etc. The growing interest of secondary metabolites in powerful biological activity outlined the need to determine their content in medicinal plants. Pyrostegia venusta (Bignoniaceae) is a neotropic evergreen vine growing a lovely ornamental plant with orange flowers cascades. This is usually present in tropical, subtropical and mild mediterranean climates. In the cool, dry season the plants form dense masses, growing trees, on walls or over rocks and are covered with flowers. Native Brazilians use decoction of P. venusta aerial parts in cough and flu treatment. The general diarrhoea, vitiligo, and jaundice control tonic. The purpose of this research is to investigate the phytochemical profile and antioxidant activity of the leaves, stems and flowers of P. venusta. Qualitative analysis of various phytochemical constituents and quantitative analysis of total flavonoids and alkaloid content were determined by the well-known test protocols available in the literature. Using standard protocols, the in vitro antioxidant activity of ethyl acetate and methanol extract of the leaves, stems, and flower was evaluated against DPPH assay method. The preliminary phytochemical analysis showed the existence of various phytoconstituents in each extract. The present study concluded that P. venusta crude extract is a rich source of secondary phytoconstituents that provide substantial antioxidant capacity. The results from this research would be of assistance to phytochemists, pharmacologists and pharmaceutical industries.