ASSOCIATION OF HIGH RISK HUMAN PAPPILOMA VIRUS SUBTYPES 16 AND 18 WITH P53 GENE POLYMORPHISMS IN ORAL SQUAMOUS CELL CARCINOMA

Abstract

HPV infections particularly the HPV types 16 and 18 have found associated with the mutations of p53 tumor suppressor gene in oral carcinoma. The present study is aimed to evaluate the role of HPV infections with the commonly found mutations of p53 gene in oral squamous cell carcinoma. The study includes 113 of control samples from healthy individuals 8 102 of test samples from the confirmed cases of oral squamous cell carcinoma. All the test and control blood samples were analysed for HPV type 16 8 18 infections and also for the polymorphisms of p53 gene by using PCRRFLP method. HPV type 18 infection was not detected in any of the control and test samples. Out of all the cases, 22.55% (23cases) of test samples 8 19.47% (22 cases) of control samples were found positive for HPV type 16 infection. HPV type 16 infections were found more prevalent in the polymorphism of codon 249 at exon 7 of p53 gene in OSCC. The result suggested that there is a significant association of HPV type 16 infections with the polymorphisms of codon 249 at exon 7 of p53 gene in OSCC.