QUANTITATIVE ANALYSIS AND ANTIMICROBIAL ACTIVITIES OF PHYLLANTHUS EMBLICA (L) LEAF EXTRACTS AGAINST FISH PATHOGEN AEROMONAS HYDROPHILA

Abstract

The present study has been to assess the qualitative screening of phytochemical constituents and quantitative study of total flavonoid content, total phenol content, total tannin and in-vitro antioxidant activity; these were determined by aluminium chloride colorimetric method, Folin-ciocalteu method, and ferric reducing antioxidant power method respectively. The anti-bacterial activity of aqueous leaf extracts of different concentration of (25mg/ml, 50mg/ml, 75mg/ml and 100mg/ml) Phyllanthus emblica against Aeromonas hydrophila was done by agar well diffusion methods. Qualitative study reported the presence of tannins, saponins, flavanoids and phenols. Quantitative investigation showed variable amounts of phytochemical constituents such as tannins, flavonoid, and phenol and antioxidant activity in the leaf extract. The most efficient inhibitory activity was examined in higher concentration (100mg/ml) of aqueous extract leaf extracts of Phyllanthus emblica. From the results, the present study indicated that Phyllanthus emblica not only contains high amount of phytochemical compounds but showed higher medicinal value. Phyllanthus emblica possess powerful antimicrobial activity against Aeromonas hydrophila and can be used in various ayurvedic treatments.