STATISTICAL OPTIMIZATION OF LACCASE PRODUCTION BY ENTEROBACTER CLOACAE STRAIN T137 UTILIZING WHEAT BRAN AS SUBSTRATE IN SUBMERGED FERMENTATION (SmF) BY PLACKETT-BURMAN METHOD

Abstract

The work aimed to produce laccase enzyme by using wheat bran as substrate by a statistical approach by Enterobacter cloacae strain T137. The study was conducted through the submerged fermentation process (SmF) by using the regression model of Plackett-Burman experiments. The significant factors influencing the laccase production was analyzed by ANOVA and Pareto method. Among the variables screened, inoculum size, trace element solution, magnesium sulfate, pH of the medium, agitation rate and incubation time found was significant when the basal salt medium was supplemented with wheat bran as the substrate. The aptness of the model was determined by the coefficient (R2) of the Plackett-Burman factorial model.