EFFECT OF ETHANOLIC EXTRACT OF COLOCASIA ESCULENTA LEAVES AND THEIR CYTOTOXICITY AND APOPTOSIS POTENTIAL ON OVARIAN CANCER CELL LINES PA-1

Abstract

In current years, the application of medicinal plants for the cancer prevention and treatment has gained more interest for the researchers due to the presence of phytoconstituents and lesser side effects. In the current finding, tropical medicinal plant Colocasia esculenta leaves was selected for its cytotoxic potential and apoptosis inducing effect against Pa-1 ovarian cancer cell lines. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-dipenyltetrazolium bromide] assays were carried out to know the cytotoxic activity and apoptosis effect was examined using flow cytometry of C. esculenta leaves of ethanol extract. The cytotoxicity was observed highest, showing the IC50 concentration at 93.2 μg/mL of the leaf extract of C. esculenta on Pa-1 ovarian cancer cell line and observed dose dependent action against the concentrations. Apoptosis inducing effect of the extract showed 63.63% of late apoptotic cells and 11.22% of early apoptotic cells of Annexin V and PI compared to the standard drug Cisplatin (15uM) showing 41.76% of late apoptotic cells and 46.42% of early apoptotic cells. C. esculenta leaves of ethanol extract have potential for development as therapeutic principle for cytotoxicity on Pa-1 ovarian cancer cell line after further investigation.