AN FTIR STUDY OF PSEUDOMONAS AERUGINOSA AVSCE-1 (KM236234) ANTAGONISM AGAINST FUSARIUM OXYSPORUM (MTCC10270) - INTERPRETATION OF FTIR DATA IN THE 4000-1000 CM-1 REGION

Abstract

Fourier transform infrared (FTIR) spectroscopy may be a rapid, reliable, sensitive, and an economical technique, which is employed as an efficient tool for microorganism identification and FTIR spectrum obtained for any identified compound gives the information on the unique fingerprint. During this study, an effort was made to interpret FTIR spectral data in differentiating the ability of antagonism of endophytic bacteria against Fusarium oxysporium var. lycopersii, a phytopathogen of tomato. Antagonism of chilli endophytic bacteria Pseudomonas aeruginosa AVSCE-1 (FR3) against Fusarium oxysporum- MTCC10270 (FO) were studied by dual culture method. FR3 showed significant antagonism against the growth of FO with inhibition of 62.5%. FTIR spectra of ethyl acetate extracts of FO, FR3 and FO+FR3 were analyzed. In FTIR analysis, C-O and C-C stretch of chitin at 1073.35 cm-1, C-N stretch of Amide II at 1232.97 cm-1, C-O stretch of Amide III at 1300 cm-1 and C=O stretch of lipid at 1650-1800 cm-1 of FO revealed the presence of pathogenic compounds. N-H stretch of peptides at 3394.21 cm-1, C-O and C-C stretch of chitin at 1073.35 cm-1, C-H stretch of alkenes at 1449.66 cm-1, C-O and C-C stretch of chitin at 1073.35 cm-1 and absence of peaks at 1500 to 2500 cm-1, in FO+FR3 and C-H stretch at 2900.83 cm-1, C=O stretch at 1732.72 cm-1, C-H stretch of unsaturated ester at 1448.73 cm-1 and C-O stretch of alkyl ether at 1097.97 cm-1 and absence C-O and C-C stretch of chitin at 1073.35 cm-1.