DEVELOPMENT AND VALIDATION OF A STABILITY INDICATING RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF HALOBETASOL AND TAZAROTENE IN BULK AND PHARMACEUTICAL FORMULATIONS

Abstract

The objective of the present study is to develop simple RP-HPLC method for the simultaneous determination of Halobetasol and Tazarotene without prior separation. In this method, Luna C18 (250 mm×4.6 mm, 5μm) column was used. The mobile phase used was methanol and 0.1 M sodium perchlorate in the ratio of 87:13 (v/v) at pH 5.8, at flow rate of 1 ml/min. UV detection was monitored at 231 nm. Calibration graphs were established in the range of 4.5 to 27μg/mL for Tazarotene and 1 to 6μg/mL for Halobetasol. The retention time for Halobetasol and Tazarotene was found to be 3.4 min and 2.6 min, respectively. The intraday and interday precision expressed as percent relative standard deviation, were below 2%. The mean recovery of paracetamol and lornoxicam was found to be in the acceptable range. Hence it can be concluded that the validated HPLC method was found to be rapid, precise and accurate and can be readily utilized for analysis of Halobetasol and Tazarotene in bulk and in pharmaceutical formulations.