OPTIMIZATION OF LACCASE ENZYMES PRODUCTION THROUGH RESPONSE SURFACE METHODOLOGY BY TRAMETES ELEGANS H6

Abstract

Laccase are multicopper oxidases containing four copper atoms per monomer distributed in three redox sites. Laccase is significant in light of the fact that it oxidizes both the harmful and nontoxic substrates. It has tremendous applications in different areas like material industry, food preparing industry, wood processing industry, drug industry, and synthetic industry. So isolation of efficient laccase producing microorganism and optimization of the process parameters for laccase production is important. Response surface methodology was employed for the optimization of different nutritional parameters for the production of laccase by the filamentous fungus Trametes elegans H6 in solid state fermentation. Initial screening of production parameters was performed using a Plackett-Burman design and the variables with statistically significant effects on laccase production were identified. Mannitol, urea and copper sulphate were found to influence laccase production significantly. These variables were selected for further optimization studies using a central composite design (CCD). The statistical optimization by response surface methodology resulted in a 1.65 fold increase in the production of laccase by Trametes elegans H6. Trametes elegans H6 can be a good source of Laccase and it can be use for industrial application.