SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS DETERMINATION OF AMILORIDE AND HYDROCHLOROTHIAZIDE IN COMBINED DOSAGE FORM

Abstract

A Simple, precise, accurate and economical spectrophotometric method was developed and validated for simultaneous estimation of Amiloride (AML) and Hydrochlorothiazide (HCZ) in combined dosage form. In simultaneous equation method, AML and HCZ were quantified using their absorptivity values at selected wavelengths, viz., 244nm and 280nm respectively in 0.1 N NaOH. The linearity range was found to be 5-25 μg/ml for AML and HCZ. The accuracy and reproducibility of the proposed method was statistically validated by recovery studies. The simultaneous equation method permits simple, rapid and direct determination of AML and HCZ in commercially available combined dosage form without previous separations and can therefore be used for routine analysis.