FORMULATION AND STANDARDIZATION OF POLYHERBAL CAPSULES FOR THE MANAGEMENT OF INFLAMMATION

Abstract

Mangifera indica and Phyllanthus emblica were extracted using 90% ethanol and 1:1, 1:2 and 2:1 ratio of the extract mixture was loaded in to liposomes. These liposomes were formulated as capsule dosage form for use in management of inflammation. The extraction yield for MI and PE were found to be 41.9 and 27.1 % respectively. The total phenolic content in MI and PE extracts were found to be 17.3±0.916 and 34.23±1.908 GAE mg/100g whereas it was found to be 49.2±2.749, 71.76±6.269 and 60.63±4.162 GAE mg/100 g for 1:1, 1:2 and 2:1 ratio mixture of MI and PE respectively. The particle size of the liposomes decreased on increasing the sonication time. The particle size of the liposome formulated using 5 min sonication time was considered optimum and was found to be 3.21 μm for blank liposome. The phenolic content in the liposomes was in harmony with the extract mixture incorporated into the liposome. EL2 exhibited the highest phenolics content. The liposomes were evaluated for stability for a period of 3 months at storage temperature of 4ºC and 40ºC. The liposomes were stable at both the temperature conditions and the particle size of the liposomes did not change significantly over a period of three months. The extract loaded liposomes at 5 min sonication time were blended with magnesium and calcium carbonates to prepare a prefill mixture for the capsules. The prefill blends were evaluated for bulk density, tapped density, angle of repose, Hausner’s ratio and Carr’s Index. All the blends possessed the capability to flow freely and may present no hindrance in capsule filling process. The weight variation of polyherbal liposome filled capsules was between 4.1-4.6 % whereas the disintegration time was found to be between 3.38 to 3.65 min.