EVALUATION OF AZO DYE ORANGE G DECOLORIZATION AND DEGRADATION BY BACTERIA ISOLATED FROM TEXTILE EFFLUENT

Abstract

The bacteria were isolated and identified from textile effluent, and their ability to decolorize the hazardous azo dye Orange G was evaluated. After incubating the isolates for 24 hours in mineral salt medium supplemented with 100 ppm Direct Orange G, their decolorizing activity was measured spectrophotometrically. Lysinibacillus sp. AUBTP7 (AK1), Bacillus muralis strain1Y118 (AK2), and Bacillus thuringiensis strain N3 (AK3) were identified as the bacterial strains. Bacillus thuringiensis strain N3 (AK3) was the most efficient bacteria at decolorizing Orange G (100ppm), removing 93% of the color in 24 hours at 36ºC temperature. Study of HPLC chromatogram of Orange G and degradation products suggests the formation of new compounds. As a result of this research, it has been revealed that some bacteria live in textile effluents and use dyes as a source of energy and nourishment, implying their usefulness in the treatment of industrial effluents.