VALIDATED RP-HPLC METHOD TO ESTIMATE EUGENOL FROM JATIPHALADI CHURNA

Abstract

Eugenol is a significant phytochemical biomarker compound of ayurvedic and other marketed herbal formulations. It shows anti-inflammatory, anti-bacterial and anti-tubercular activity. Thus, it is a suitable bioactive constituent to establish the quality of commercial drug and its formulation. The aim is to develop and validate an efficient and effective RPHPLC method for quantification of eugenol from commercial marketed formulations. Separation was carried out on a cosmosil C18 column, mobile phase composition was methanol: distilled water (60:40, v/v) at flow rate of 1mL/min. Detection was carried out at 215 nm using a photodiode array detector (PDA). Commercial Ayurvedic formulation such as Jatiphaladi Churna was further subjected to RP-HPLC for estimation of eugenol. The RP-HPLC method was validated as per ICH guidelines. The LOD and LOQ level were found to be 25.00ng/mL and 50.00 ng/mL, respectively. Detector response was found to be linear from 50.00ng/mL to 50,000.00 ng/mL. The method was found to be simple, accurate, reproducible and rugged. This method was used to estimate the content of eugenol in commercial formulations and the method is found suitable for quality assurance and marker based standardization of ayurvedic formulations containing eugenol.