PHYTOCHEMICAL SCREENING, ESTIMATION OF TOTAL PHENOLIC, FLAVANOID CONTENT AND ESTIMATION OF ANTIOXIDANT ACTIVITY OF HYDROALCOHOLIC EXTRACT OF VITEX NEGUNDO, VITEX TRIFOLIA AND VITEX PARVIFLORA

Abstract

The objectives of this study are to screen the phytochemicals, estimate the content of phenolic and flavonoid compounds and determine the antioxidant capacity of the leaves hydroalcoholic extract of Vitex negundo, Vitex trifolia and Vitex parviflora. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenol and flavonoids were determined by the well-known test protocol available in the literature. The hydro alcoholic extract of bark of leaves extract of Vitex negundo, Vitex trifolia and Vitex parviflora was studied for antioxidant activity on In vitro models namely 1,1-diphenyl, 2-picryl hydrazyl (DPPH) assay. Phytochemical analysis revealed the presence of phenols and flavonoids in all the three plants. TPC of hydroalcoholic extract of Vitex negundo, Vitex trifolia and Vitex parviflora showed the content values of 0.872, 0.784 and 0.463 mg/100mg of dried extract respectively followed by TFC were found 0.974, 0.657, 0.652 mg/100mg of dried extract in hydroalcoholic extract of Vitex negundo, Vitex trifolia and Vitex parviflora respectively. DPPH radical scavenging assay measured hydrogen donating nature of extracts. Under DPPH radical scavenging activity, the inhibitory concentration 50% (IC50) value of Vitex negundo, Vitex trifolia and Vitex parviflora hydroalcoholic leaves extracts were found to be 50.33μg/ml, 70.57μg/ml and 73.05μg/ml as compared to that of ascorbic acid.