A NOVEL AND RAPID HPTLC METHOD FOR THE ANALYSIS OF CITALOPRAM HYDROBROMIDE IN TABLET DOSAGE FORM – DEVELOPMENT AND VALIDATION

Abstract

Aim of the present investigation was to develop and validated a new, simple, sensitive, selective and precise High Performance Thin Layer Chromatograpic (HPTLC) method for the determination of Citalopram HBr in tablet dosage form. TLC aluminium sheets pre coated with silica gel 60F-254 were used as the stationary phase and Methanol:Water:Ethyl acetate (4:2:4) was used as the mobile phase for the linear ascending development carried out in twin trough glass chamber at room temperature (25±20C). Spectrodensitometric scanning and analysis in absorbance mode at 240nm were carried out using CAMAG TLC Scanner 3. Compact spots for Citalopram HBr were observed having Rf value of 0.46±0.02. Linear regression analysis of the data for the calibration plots showed good linear relationship in the concentration range of 2-12mcg/spot with respect to peak area and r value was found to be 0.9960. The method was validated as per ICH guidelines for accuracy calculated as % recovery was in the range of 98.63% to 101.72%. The statistical analysis of the data showed that the method is reproducible and selective for the estimation of Citalopram HBr in tablet dosage form during routine analysis.