DEVELOPMENT OF TRIAZINE BASED DENDRIMER OF SALICYLIC ACID WITH INCREASED ANTIBACTERIAL ACTIVITY

Abstract

The aim of this research work was to develop triazine based dendrimer of salicylic acid with increased antibacterial activity. Surface modification of dendrimer of generation 3 was performed by conjugation with polyethylene glycol (PEG). The amount of drug encapsulated in dendrimer was determined by the separation of drug loaded dendrimer from the suspension containing free drug by centrifugation. The suspension obtained after solvent evaporation was centrifuged. The amount of free drug in the supernatant was measured by UV spectrophotometer. The amount of drug entrapped into dendrimer was calculated as the difference between the drug used for formulation and the amount of drug in supernatant. Antibacterial activity shows promising results as prepared dendrimer drug conjugate shows better results with only 36.05 % entrapped drug as compared to standard drug. 36.05 % entrapped drug gives results more than as compared to standard drug sample. Results reveals that if we assume that the standard drug is completely entrapped, there will be approximately 2.5 fold increase in antibacterial activity. This study also reveals that salicylic acid and prepared dendrimer drug conjugates show better activity for S. aureus i.e. for gram positive bacteria.