OPTIMUM ACTIVITY TEMPERATURES OF FPASES FROM PSYCHROTOELRENT (PENICILLIUM CANESCENS AND RHODOTORULA MUCILAGINOSA) AND PSYCHROPHYLIC (PSEUDOGYMNOASCUS ROSEUS) FUNGI

Abstract

FPAses have been isolated and studied from psychrotolerant yeast Rhodotorula mucilaginosa BPT1, Penicillium canescens BPF4 and Pseudogymnoascus roseus BPF6. BPT1 showed 100% activity at 4ºC, 30ºC and 50ºC, while that from BPF4 and BPF6 showed maximal activity at 60ºC and 40ºC respectively. The enzyme from BPT1 showed three peak activities, BPF4 and BPF6 showed single peak activity. While BPT1 FPAse showed 100% activity at low temperature i.e. 4ºC, rendering it very useful enzyme. The FPAses from both the other fungi also showed more than 60% residual activity at cold temperatures. The cold-activity of the enzymes makes them potential for application in simultaneous saccharification and fermentation and other industries especially food processing ones.