EVALUATION OF IN-VITRO LITHOLYTIC ACTIVITY OF PLANT BOUGAINVILLEA SPECTABILIS

Abstract

The aim of present investigation is to evaluate the in-vitro anti urolithiatic activity of the leaves of the plant Bougainvillea spectabilis (Willd.). Ethanolic and aqueous extracts of Bougainvillea spectabilis fresh leaves were prepared. Homogenous precipitation methods were used to prepare artificial stones of calcium oxalate. Semi-permeable membrane of eggs was used as dissolution bags. Different in-vitro anti urolithiatic studies were used such as method of mineralization and titrimetric method to evaluate the inhibition and dissolution of stone crystal using standard drug Cystone. In inhibition as well as dissolution models, the ethanolic extract of Bougainvillea spectabilis has shown better capability to dissolve calcium oxalate. Cystone exhibited strongly inhibitory action in both, the method of mineralization and titrimetric assay. Thus, it was concluded that the ethanolic extract of Bougainvillea spectabilis exhibited inhibitory action in both of the methods to a significant level and the bioactive natural products present in leaf extracts of Bougainvillea spectabilis can be used in the development of new pharmaceuticals that enhances therapeutic use in renal calculi.