GREEN SYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES USING ETHANOLIC EXTRACT OF MIMOSA PUDICA LINN LEAVES

Abstract

Green synthesis of metal nanoparticles using plant extract is an eco-friendly and cost-effective method for synthesizing metal nanoparticles. In this present work, silver nanoparticles have been synthesized using ethanolic extract of Mimosa pudica Linn plant (MPL) leaves. Green synthesized silver nanoparticles (AgNps) have been characterized by UV-Vis spectrometer, Fourier Transform Infra-Red ((FTIR) spectra, dynamic light scattering (DLS), Scanning Electron Microscopy (SEM) and X-ray diffraction (XRD) methods. UV-Vis spectrum of synthesized silver nanoparticles from Mimosa pudica Linn leaves extract shows a characteristic an absorption peak at 421 nm. FTIR analysis revels that presence of silver nanoparticles the presence of some biomolecules in extracts that act as reducing and capping agent for green synthesis of silver nanoparticles. DLS analysis showed AgNPs were drifted widely from 58.6 to 157.7 nm, with an average particle size of 104.7 nm. The particles are found to be polydisperse and slightly agglomerated due to the presence of phytochemicals present in the plant extract. Scanning electron microscope showed silver nanoparticles are spherical shaped. The X-RD pattern revealed the presence of crystalline, dominantly spherical silver nanoparticles in the sample having size ranging from 42 to 50 nm. The XRD peaks 38.08°, 44.22°, 64.42°, and 77.32° for leaves extract and 38.1°, 44.3°, 64.5°, 77.5°, and 81.33° for callus extract can be assigned the plane of silver crystals (111), (200), (220), and (311), respectively, and shows face-centered, cubic and crystalline nature of the silver nanoparticles. The green synthesized silver nanoparticles showed significant antibacterial activity.