BIO-MINERALIZATION OF DISPERSE DYE AZO ORANGE -3 BY AN ALKALIPHILE PSEUDOMONAS DL 17

Abstract

Pseudomonas DL 17, isolated from hyper saline Lake Lonar (MS) Buldana, India was tested for complete mineralization of azo dye and other nitro-aromatics. After adapting for several days it was used for bio-degradation of Disperse orange 3 where it’s experimental concentration was completely mineralized within 24 hrs. Solvent extracted metabolites were characterized as p-nitroaniline, p-phenylenediamine, acetanilide, catechol etc. On the basis of UV-Vis., FTIR, NMR, GCMS analysis, mechanistic pathway for biodegration was designed. CYP450 and other related enzymes involved in biodegradation were found hiked comparative to standard. Azo reductase, Nitro reductase, Tyrosinase, and SOD showed more activity than other enzymes under study.