ISOLATION OF FISETIN 3-O-GLUCOSIDE FROM VITEX NEGUNDO AND ITS HYPOTONICITY INDUCED MEMBRANE STABILIZATION STUDIES

Abstract

The attractive violet flowers of Vitex negundo.-Linn of Verbenaceae family are very popular for its pharmacological properties. Literature survey reveals that Vitex negundo was a frequently used traditional plant for its high medicinal properties. The plant can be further explored for the study of traditionally claimed unexplored activities, as well as isolation and identification of active constituents may lead to new findings. In the present study, the flowers of Vitex negundo were collected, dried and extracted with various solvents according to solubility index, and finally five extracts were studied. Finally, the isolated compound was identified as fisetin 3-o-glucoside. It was confirmed by thin layer chromatography (TLC), UV and NMR studies and the isolated compound were used to undergo hypotonicity induced membrane stabilization studies.