BIOFILM INHIBITION POTENTIAL OF AQUEOUS LEAF EXTRACT OF SARACA ASOCA LINN. AGAINST PSEUDOMONAS AERUGINOSA

Abstract

About 95-99% of microorganisms exist in nature as biofilms. The homogenous or heterogeneous communities of pathogenic bacteria constituting microbial biofilms exert anti-microbial resistance. Several natural anti-biofilm agents have been recognized, like phytochemicals, biosurfactants, anti-microbial peptides, and microbial enzymes. The present study investigates the effect of aqueous extract of Saraca asoca Linn. on the in vitro formation of pseudomonas aeruginosa biofilm. The quantitative assessment of biofilm formation was done by crystal violet staining assay. Various factors such as temperature, pH, and concentration were optimized for enhanced effect in inhibiting the biofilm by aq. Saraca asoca leaves extract. Aq. Saraca asoca leaves extract was characterized using GC-MS analysis. Brine shrimp lethality bioassay was also carried out to investigate the cytotoxicity of aq. extract. Saraca asoca was observed as a potential source of anti-biofilm molecules and can be further harnessed to maximum potential.