PHYTOGENIC SYNTHESIS OF SILVER NANOPARTICLES USING IPOMOEA OBSCURA (L) LEAVES EXTRACT AND ITS FUNGICIDAL ACTIVITY AGAINST PHYTOPATHOGENS

Abstract

The current study was carried out to synthesize the silver nanoparticles using leaf extract of Ipomoea obscura through green
chemistry approach. Synthesis of silver nanoparticles were confirmed by the change in colour of reaction mixture from
pale yellow to dark brown and absorption band obtained at 430 nm by UV-visible spectroscopic studies. Characterization
of synthesized nanoparticles was carried out by XRD, DLS, FTIR, SEM and EDAX for their structure, size, possible
functional groups, shape and elemental composition respectively. The XRD spectrum of the synthesized silver
nanoparticles showed three distinct diffraction peaks at 2θ values 37.89°, 43.96° and 64.32° corresponding to lattice
planes (111),(200) and (220) respectively confirming that the particles are face centred cubic in nature. The dynamic
light scattering spectroscopic (DLS) analysis showed that the particles are 46nm in size. SEM images of nanoparticles
revealed the cubic shape of particles and the Energy Dispersive X-ray analysis (EDAX) confirmed the significant presence
of elemental silver. Synthesized silver nanoparticle showed good fungicidal activity against Didymella bryoniae, Fusarium
oxysporum, Fusarium moniliforme and Aspergillus flavus.