DETERMINATION OF METRONIDAZOLE IN BIOLOGICAL SAMPLES EMPLOYING DIFFERENTIAL PULSE AND SQUARE WAVE POTENTIAL-WAVEFORMS STRIPPING VOLTAMMETRIC METHODS

Abstract

Metronidazole is an anti-microbial drug. In the pharmaceutical industry, Electro chemical analysis is an effective
analytical method that is being more widely used. In Cyclic voltammetry glassy carbon electrodes is very crucial for
the determination of pharmaceuticals. This research may lead to the establishment of a highly efficient analytical
approach for determining Metronidazole in pharmaceutical as well as biological samples. Cyclic voltammetric
investigation of Metronidazole tablets was carried out by using a three-electrode cell system, which were activated
glassy carbon electrode as working electrode, Ag/AgCl as the reference electrode and a platinum wire as the auxiliary
electrode for the analysis of Urine and Blood serum samples. Metronidazole exhibited one distinct and well-defined
cathodic peak in the potential range -0.5 to -1Vvs Ag/AgCl/KCl reference electrode, at all concentrations. Mean
percentage recoveries and relative standard deviations of 98.54 (1.117) (DP-CAdSV) and 99.54 (1.541) (SW-CAdSV)
were achieved based on replicate measurements of 1×10-6 mol L-1 spiked human urine. Similarly on replicate
measurements of 2x10-6mol L-1 Metronidazole in spiked human serum, mean percentage recoveries and relative standard
deviations of 99.95 (0.722) (DP-CAdSV) and 99.69 (1.238) (SW-CAdSV) were obtained This procedure requires no
pre-treatment, sensitive and time saving therefore it can be adopted for the pharmacokinetic studies.