IMPACT OF REGIONAL VARIATION ON LUPEOL CONTENT IN CARISSA CARANDAS LINN. FRUITS: EVALUATION USING VALIDATED HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY

Abstract

The present work is an attempt to evaluate the impact of regional variation on lupeol content in Carissa carandas Linn. fruits collected from different geographical regions of India using a validated HPTLC method. Chromatographic separation was achieved on TLC plates pre-coated with silica gel 60 F254 using toluene: methanol (8:1, v/v) as a mobile phase. Detection of lupeol was carried out by derivatizing the plates with 10 % methanolic sulphuric acid reagent followed by its densitometric scanning using CAMAG TLC scanner 4 at 366 nm. The method was validated as per ICH guidelines. Statistical analysis reveals that, the content of lupeol in different geographical region varied significantly.