PRODUCTION OF LACCASE FROM GANODERMA SP. BY USING AGRO-RESIDUAL WASTES

Abstract

Solid-state fermentation (SSF) has emerged as a potential technology for the production of microbial products such as
feed, fuel, food, industrial chemicals and pharmaceutical products. Its application in bioprocesses such as bioleaching, bio
beneficiation, bioremediation, biopulping, etc. has offered several advantages. Utilisation of agro-industrial residues as
substrates in SSF processes provides an alternative avenue and value-addition to these otherwise under- or non-utilised
residues. The aim of this study was to determine the most promising fungus, the best solid substrate and the optimal
conditions for the production of laccase. A laccase producing fungus Ganoderma sp. was isolated and produces laccase by
utilizing the agro-wastes. The study revealed that the rice bran was found to be the best supported lignocellulosic
substrate for extracellular laccase production under SSF and optimum condition for highest lacacse activity of pH and
temperature were observed at 6.0 and 45°C respectively. The incubation periods for laccase maximum activity was at 8th
day. Glucose and Peptone were the best supported carbon and nitrogen source for the maximum laccase activity.