PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT ACTIVITY OF WHOLE PLANT OF TRIDAX PROCUMBENS LINN.

Abstract

Antioxidants serve a critical role in preventing oxidative stress-related damage. Plants with high phenolic content have
been shown to have antioxidant effects. The goal of this study was to look into the phytochemistry and antioxidant
properties of ethanolic extracts from Tridex procumbens (whole plant) in order to establish a statistical link between
isolated component concentration and antioxidant capability. TLC, HPTLC, Column chromatography, and an in vitro
standard technique employing a spectrophotometer were used to assess the phytochemistry and antioxidant properties of
ethanolic extractives. DPPH (l, l-diphenyl-2-piciylhydrazine) radical scavenging assay, Nitric oxide (NO) Radical
Inhibition Assay, Reducing Power and hydroxyl radical scavenging assay techniques were used to assess antioxidant
activity. TLC plate with various numbers of 5 spots were implemented. The HPTLC report shows the existence of ten
spots with distinct Rf values, indicating that they occupy separate areas with varying Rf values. The extracted chemicals
from the ethanolic extract of Tridex procumbens were column chromatographed using hexane (80ml) and benzene (20 ml)
and a stationary phase silica gel. The maximum antioxidant activity was found in extracts. The Tridax procumbens extract
was the most efficient in terms of DPPH and hydroxyl radical scavenging activities, with IC50 values of 0.15, 0.12, 0.008
and 42.39 g/ml, respectively. The findings show a strong link between separated chemical content and extract
antioxidant capability, suggesting that Tridex procumbens might be a promising option for medicinal plant-based products as
a free radical inhibitor or scavenger. However, further research is needed to ensure that it can be used effectively in both
modern and traditional medicine.