EVALUATION OF IMMUNOMODULATORY ACTIVITY OF ETHANOLIC EXTRACTS OF MORINGA CONCANENSIS NIMMO IN MICE

Abstract

The present study was undertaken to evaluate immunomodulatory activity of ethanolic whole plant extract of Moringa concanensis and isolated compound from Moringa concanensis. Immunomodulatory activities were determined by in vivo models- delayed-type hypersensitivity (DTH), carbon clearance assay (phagocytic index) and cyclophosphamide induced immunosupression. The ethanolic extracts of Moringa concanensis and isolated compound, in the concentration range 5,10,25,50, and 100 μg were also tested for phagocytic effect on human neutrophils using the in vitro models–nitroblue tetrazolium (NBT) dye test. Mice treated with dose i.e. 170 mg/kg (Group III) and dose 1 g/kg (Group IV) increase response in foot pad edema was found to be statistically significant (*p less than 0.05), (**p less than 0.01) and showed statistically significant (p less than 0.05) increase in WBC and platelets count and Phagocytosis index when compared to Cyclophosphamide (20 mg/kg) treated and control treated mice. In cyclophosphamide induced immunosupression, ethanolic roots extract Moringa concanensis and isolated compound showed significant (P less than 0.001) increase in total differential leucocytes count DLC and hematological parameters RBCs, Hb, Platelets and WBCs significant increases when compared with cyclophosphamide group. Ethanolic extract of Moringa concanensis and isolated compound in the concentration range 5, 10, 25, 50 and 100 μg, also showed significant (P less than 0.001) phagocytics effect on human neutrophils in the parameters studied. The present experimental finding demonstrated that isolated compound has superior immunomodulatory activity then ethanolic extract of Moringa concanensis, this effect was presumably due to greater ability of isolated compound to boost the innate and adaptive immune system.