IN-VITRO EVALUATION OF IMMUNOMODULATORY ACTIVITY OF METHANOLIC EXTRACT ROOTS OF PAVONIA ODODRATA

Abstract

The present study was undertaken to evaluate the immunomodulatory activity of methanolic roots extract of Pavonia odorata and isolated compound from Pavonia odorata. Immunomodulatory activities were determined by in vitro modelsplaque- forming cell assay, nitric oxide (NO) radical scavenging activity, inhibit RAW 264.7 macrophage cell line from generating harmful NO induced by lipopolysaccharide (LPS) and MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. The number of antibodies secreting cells from spleen was determined using plaque-forming cell assay. The effect of plaque-forming by methanolic and isolated compound of Pavonia odorata showed significant (P less than 0.01) compared to control showed significant (P less than 0.001) phagocytic effect on human neutrophils in the parameters studied. Scavenged nitric oxide radical by methanolic extract of PO inhibition 38% and isolated compound scavenged nitric oxide radical inhibtion 40% respectively compared to control group exerted 52% decrease of nitric oxide radical respectively concentration at 0.1,0.01 and1.0 mg/ml. LPS in murine macrophage, RAW 264.7 cells culture medium induced a high release of NO but pretreatment with Pavonia odorata methanolic extract and isolated compound resulted in inhibition of NO production by 22, 26, 99 % and 28, 36, 96% at concentrations of 0.5, 0.1 and 1.0 mg/ml, compared to LPSinduced sample. Methanolic extract and isolated compound significantly stimulate RAW 264.7 cell viability (P less than 0.001) by MTT assay. The present experimental finding demonstrated that isolated compound has superior immunomodulatory activity than the methanolic extract of Pavonia odorata this effect presumably due to the greater ability of the isolated compound to boost the innate and adaptive immune system.